LC-MS/MS analysis of 2-aminothiazoline-4-carboxylic acid as a forensic biomarker for cyanide poisoning

Figure 5 Endogenous level of 2-aminothiazoline-4-carboxylic acid in mice organs. The endogenous levels of 2-aminothiazoline-4-carboxylic acid (ATCA) in different organs of mice were measured using the solid phase extraction procedure and LC-MS/MS conditions outlined above. Triplicate samples from healthy mice showed endogenous ATCA concentrations as follows: 1.2 ± 0.1 μg/g for kidney samples, 1.6 ± 0.1 μg/g for brain samples, 1.8 ± 0.2 μg/g for lung samples, 2.9 ± 0.1 μg/g for heart samples, and 3.6 ± 0.9 μg/g for liver samples.