LC-MS/MS analysis of 2-aminothiazoline-4-carboxylic acid as a forensic biomarker for cyanide poisoning

Figure 3 Comparison of chromatograms of 2-aminothiazoline-4-carboxylic acid and 2-aminothiazole-4-carboxylic acid obtained by using 0. 5% acetic acid/MeOH as mobile phase (A and C) and 0.5% trifluoroacetic acid/MeOH as mobile phases (B and D). When trifluoroacetic acid (TFA) was added to the mobile phase a good sharp 2-aminothiazole-4-carboxylic acid (ATZA) peak was detected. This result suggested that TFA was a more effective ion-paring reagent than acetic acid during the chromatography. Therefore, 0.5% TFA/MeOH was selected as the mobile phase, replacing the 0.5% acetic acid/MeOH mobile phase used in our prior work. ATCA: 2-aminothiazoline-4-carboxylic acid.