LC-MS/MS analysis of 2-aminothiazoline-4-carboxylic acid as a forensic biomarker for cyanide poisoning

Figure 2 Comparison of 2-aminothiazoline-4-carboxylic acid and 2-aminothiazole-4-carboxylic acid signals detected by LC-MS/MS before and after evaporation of solvents by air or argon stream. The decreased peak areas of 2-aminothiazoline-4-carboxylic acid (ATCA) and 2-aminothiazole-4-carboxylic acid (ATZA) in reconstituted solutions following air or argon drying were attributed to the variations of trifluoroacetic acid concentration in the stock solution and the freshly prepared mobile phase. Solvent evaporation with argon had similar effects to that with air. Therefore, the use of an inert gas was not needed for solvent evaporation.