Selective procedure for the instant identification of cellular apoptosis induced by natural products

Figure 3 Pathological changes of LoVo cells after treatment with 140 µg/mL Pinus massoniana bark extract for 24 hours or not by flow cytometry analysis. A: Control group, a normal cell cycle distribution of an untreated LoVo cell line stained with propidium iodide (PI), there is no obvious apoptotic curve- sub-G1 curve but with a percentage of 10.9% spontaneous apoptosis. Treatment group, altered cell cycle distribution of a treated LoVo cell line stained with PI, there is an obvious apoptotic curve-sub- G1 curve before G1 curve with a gross percentage of 31.4% induced apoptosis; B: Statistical histogram of LoVo cell apoptosis induced by PMBE treatment compared with Control. ^aP < 0.05 vs Control, n = 3, mean ± SD. C: Control group; T: Treatment group.