Selective procedure for the instant identification of cellular apoptosis induced by natural products

Figure 1 Fluorescence microscopy observation of LoVo cell apoptosis. Propidium iodide staining of LoVo cells untreated and treated with 140 µg/mL Pinus massoniana bark extract for 24 hours under fluorescence microscope with G.B. filter. Normal cells depicted in the panel show light and homogeneous staining of their nuclei. In contrast, apoptotic cells represented in the panel show thick and irregular staining of their nuclei as a result of chromatin condensation and nuclear fragmentation. Magnification, 400 ×. N: Normal cells; A: Apoptotic cells; C: Control group; T: Treatment group.