Time-dependent impact of a high-fat diet on the intestinal barrier of male mice

Figure 3 Immunohistochemical staining for occludin in the ileum and transmission electron microscopy of the ileum. A: Occludin immunostaining is shown in light brown (open arrow) and was decreased in a time-dependent manner in the high-fat (HF)-fed groups. B: Transmission electron microscopy images showing normal ileal ultrastructures in the control diet for 10 wk and control diet for 16 wk groups, with preserved tight junctions (asterisk). In contrast, the HF diet for 10 wk and HF diet for 16 wk groups exhibited disrupted tight junctions (open arrow) in addition to a damaged ultrastructure. C10: Control diet for 10 wk; C16: Control diet for 16 wk; HF10: High-fat diet for 10 wk; HF16: High-fat diet for 16 wk.