Time-dependent impact of a high-fat diet on the intestinal barrier of male mice

Figure 2 Staining of goblet cells with alcian blue, Q_A (goblet cells), relative Mucin2 mRNA expression, and plasma lipopolysaccharide concentrations. A: Large intestine tissue stained with alcian blue and periodic acid-Schiff (PAS), revealing that the goblet cells were stained blue; B: Numerical density of goblet cells per area [Q_A (goblet cells)]. Alcian blue and PAS stained glycoproteins produced by goblet cells (scale bar = 40 μm) were markedly increased in the high-fat diet for 10 wk group according to stereology [Q_A (goblet cells)]. ; C: Relative Mucin2 mRNA expression in different groups; D: Plasma lipopolysaccharide concentrations in different groups. Brown-Forsythe and Welch one-way ANOVA and Dunnett T3 post hoc test were used (mean ± SD, n = 5). ^aP < 0.01, compared with control; ^bP < 0.01, compared with high-fat diet for 10 wk; ^cP < 0.01 compared with control diet for 16 wk. C10: Control diet for 10 wk; C16: Control diet for 16 wk; HF10: High-fat diet for 10 wk; HF16: High-fat diet for 16 wk; LPS: Lipopolysaccharide.