Time-dependent impact of a high-fat diet on the intestinal barrier of male mice

Table 1 Primers used for determination of the phylum or class of microorganisms of the gut microbiota

Phylum or class	Forward primer	Reverse primer
Actinobacteria	5’-TACGGCCGCAAGGCTA-3’	5’-TCRTCCCCACCTTCCTCCG-3’
Bacteroidetes	5’-CRAACAGGATTAGATACCCT-3’	5’-GGTAAGGTTCCTCGCGTAT-3’
Class-γ-proteobacteria	5’-TCGTCAGCTCGTGTYGTGA-3’	5’-CGTAAGGGCCATGATG-3’
Eubacteria (all bacteria)	5’-ACTCCTACGGGAGGCAGAGT-3’	5’-ATTACCGCGGCTGCTGGC-3’
Firmicutes	5’-TGAAACTYAAAGGAATTGACG-3’	5’-ACCATGCACCACCTGTC-3’