Urine exosome mRNA-based test for monitoring kidney allograft rejection: Effects of sample transportation and storage, and interference substances

doi:10.5662/wjm.v13.i5.492

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World Journal of Methodology

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2222-0682

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Methodol. Dec 20, 2023; 13(5): 492-501
Published online Dec 20, 2023. doi: 10.5662/wjm.v13.i5.492

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Figure 1 Stability of exosomal mRNA upon urine transportation at different temperatures, and effects of various urine upfront processing techniques. A: Donor 1; B: Donor 2; C: Donor 3; D: Donor 4. For each donor, levels of 9 mRNA targets were analyzed by quantitative polymerase chain reaction; the graph displays Ct values (y-axis). Conditions mimicking urine transportation and handling, left to right: 2 d at +4C; 2 d at +20C; 2 d at +40C; freeze/thaw; 2x freeze/thaw; urine freeze/thaw without pre-processing.

Citation: McFaul M, Ventura C, Evans S, Dundar H, Rumpler MJ, McCloskey C, Lowe D, Vlassov AV. Urine exosome mRNA-based test for monitoring kidney allograft rejection: Effects of sample transportation and storage, and interference substances. World J Methodol 2023; 13(5): 492-501
URL: https://www.wjgnet.com/2222-0682/full/v13/i5/492.htm
DOI: https://dx.doi.org/10.5662/wjm.v13.i5.492