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©The Author(s) 2023.
World J Methodol. Dec 20, 2023; 13(5): 484-491
Published online Dec 20, 2023. doi: 10.5662/wjm.v13.i5.484
Published online Dec 20, 2023. doi: 10.5662/wjm.v13.i5.484
Figure 3 Identification of the quality of the total proteins isolated from the same cultured HepG2 cells.
The total proteins were separated on 12% gels by SDS-PAGE (Lane 1). Following transfer onto a polyvinylidene fluoride membrane, the 40-kDa GAPDH-specific band was blotted with HRP-anti-GAPDH and observed using sediment type mono-ingredient TMB substrate solution (Lane 2).
- Citation: Cui YY. Sequential extraction of RNA, DNA and protein from cultured cells of the same group. World J Methodol 2023; 13(5): 484-491
- URL: https://www.wjgnet.com/2222-0682/full/v13/i5/484.htm
- DOI: https://dx.doi.org/10.5662/wjm.v13.i5.484