Sequential extraction of RNA, DNA and protein from cultured cells of the same group

Figure 2 Identification of genomic DNA and determination of the quality of the total RNA isolated from cultured HepG2 cells. Electrophoresis of the genomic DNA and products of reverse transcription (RT)-polymerase chain reaction (PCR) amplification of the c-Myc fragment on a 2.0% agarose gel and subsequent staining with 0.5 mg/mL ethidium bromide revealed the presence of 1.0-20 kb genomic DNA fragments (Lane 1), and a 500 bp c-Myc fragment that was amplified by RT-PCR using the total RNA as the template (Lane 2).