Role of nestin in glioma invasion

Figure 1 Effect of nestin knockdown on cell migration. A: Top, cartoon illustrating the experimental design (described in details in materials and methods). Bottom, the two-dimensional area spread of a selected HSR-GBM1 sphere is shown; B: Two dimensional area spread analysis shown no significant difference in cell migration for HSR-GBM1 (left) uninfected, shC, and shN infected cells. In contrast, a significant reduction in cell migration was observed for shN infected HSR040622 cells (right) as compared with uninfected and shC infected cells (NS: Not significant, ^bP < 0.001; two sided t test). shC: Scrambled control.