Pro- vs anti-stenotic capacities of type-I vs type-II human induced pluripotent-derived endothelial cells

Figure 3 Histological analyses after three weeks. Histologies of WI-operated femoral arteries after three weeks from PVVT in mice regularly administrated with aAGM1 were examined. A: Photographs of HE-stained samples at low magnification; B: Stenosis indexes were calculated and statistically analyzed by student-t test. Data were presented as average (Av) ± standard deviation (SD). Experiments were performed using three mice for each condition (n = 3); C and D: High magnification photographs of the samples with HE staining (C, left), immunostaining using anti-smooth muscle actin (SMA) antibody with nuclear counterstaining by DAPI (C, right) and immunostaining using anti-hPECAM1antibody with DIC and nuclear counterstaining by DAPI (D). WI: Wire injury; iPSC: Induced pluripotent stem cell; VEC: Vascular endothelial cell; iPSdECs: iPSC-derived VECs; αAGM1: Anti-asialo GM1 monoclonal antibody.