p38 mitogen-activated protein kinase regulates type-I vs type-II phenotyping of human vascular endothelial cells

Figure 4 Interaction between sphingosine-1-phosphate receptors and regulator of G-protein signaling 5 in type-I vascular endothelial cells. A and B: Immunoprecipitation (IP) followed by Western blotting (WB) was performed using HUVEC lysates and indicated antibodies; C: Human aortic smooth muscle cells were subjected contact co-culture experiments with type-II VECs with or without 1 nmol/L FTY720-P (n = 3, AV ± SD). HUVEC: Human umbilical vein endothelial cells; VECs: Vascular endothelial cells; SeV-iPS: Sendai virus vector-based induced pluripotent stem cells; RGS5: Regulator of G-protein signaling 5.