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©2014 Baishideng Publishing Group Inc.
World J Transl Med. Aug 12, 2014; 3(2): 69-83
Published online Aug 12, 2014. doi: 10.5528/wjtm.v3.i2.69
Published online Aug 12, 2014. doi: 10.5528/wjtm.v3.i2.69
Table 2 Advantages and disadvantages of different platelet function methodologies
Platelet tests | Advantages | Disadvantages |
Bleeding time | Physiological In vivo test Easy, quick No WB processing | Operator dependent Invasive Poorly standardized Dependent on different variables (skin thickness, t°C) |
Light transmission platelet aggregation in PRP | Historical gold standard Flexible Diagnostic method Different agonists available Sensitive for anti-plt therapy | Pre- and analytic variables Time-consuming High sample volume Sample preparation |
WB Impedance Platelet Aggregometry | No sample preparation Flexible Diagnostic method Different agonists available Sensitive for anti-plt therapy Close to POCT (Multiple system) | Limited HCT and platelet count range |
Flow Cytometry | Small blood volumes Diagnosis ex vivo of platelet activation Evaluation of efficacy of thienopyridyne therapy | Expensive Specialized equipment Experienced operator Careful sample processing Probable, possible artifacts |
Platelet Function Analyzer -PFA-100 /Innovance PFA-200 | In vitro standardized BT POCT Easy, quick Sensitive to severe platelet dysfunctions | Nonflexible Platelet count- HCT-dependent Not sensitivity for platelet secretion defects. |
VerifyNow system | POCT WB assay Easy, quick No WB processing | Expensive Nonflexible Monitoring antiplatelet therapy only Limited HCT and platelet count |
Impact Cone and Plate(let) analyzer | WB assay Global platelet function Small sample volume | Expensive Experienced staff Lacking of clinical studies Not widely available |
Plateletworks | POC WB system Minimal sample preparation Easy, rapid screening test | Indirect assay Required platelet count method Not so well studied |
Viscoelastic methods | POCT Global hemostasis test Anticoagulation monitoring Predicts bleeding Reduces blood transfusions Improve clinical outcome | Measure clot properties Depend on: platelet function, coagulation and fibrinolysis factors More studies are needed |
- Citation: Paniccia R, Priora R, Liotta AA, Maggini N, Abbate R. Assessment of platelet function: Laboratory and point-of-care methods. World J Transl Med 2014; 3(2): 69-83
- URL: https://www.wjgnet.com/2220-6132/full/v3/i2/69.htm
- DOI: https://dx.doi.org/10.5528/wjtm.v3.i2.69