Copyright
©2014 Baishideng Publishing Group Inc.
World J Transl Med. Aug 12, 2014; 3(2): 69-83
Published online Aug 12, 2014. doi: 10.5528/wjtm.v3.i2.69
Published online Aug 12, 2014. doi: 10.5528/wjtm.v3.i2.69
Table 1 Laboratory and point-of-care assays for evaluation of platelet function
Platelet function tests | Principle of method | Application of the methods |
Platelet adhesion studies | ||
Bleeding time | In vivo stopping of blood flow | Screening test of platelet function on defects of primary hemostasis |
Platelet Function Analyzer - PFA-100/InnovancePFA-200 | In vitro stopping of high shear blood flow by platelet plug in whole blood | Assessment of bleeding risk, thrombotic risk, drug effects Sensitive to severe platelet dysfunctions Detection of VWD |
Impact Cone and Plate(let) analyzer | Shear-induced platelet adhesion/aggregation onto surface in whole blood | Screening of congenital primary hemostasis abnormalities Evaluation of platelet response to aspirin and clopidogrel (scarce data). |
Platelet-To-Platelet Aggregation Studies | ||
Light transmission platelet aggregation | Low shear platelet-to-platelet aggregation in response to agonists in platelet-rich-plasma | Screening test for bleeding behavior Diagnostic for platelet surface glycoprotein defects Monitoring of the platelet response to antiplatelet agents |
Impedance platelet aggregation | Low shear platelet-to-platelet aggregation in response to agonists in whole blood | Screening test for bleeding behavior Diagnostic for platelet surface glycoprotein defects Monitoring of the platelet response to antiplatelet agents |
VerifyNow system | Fibrinogen-platelet agglutination in response to agonist in whole blood | Monitoring of the platelet response to antiplatelet agents |
Plateletworks | Platelet counting pre- and post-activation in whole blood | Monitoring of the platelet response to antiplatelet agents |
Analysis of Clot Formation | ||
Thromboelastography/ Thromboelastometry | Monitoring of rate and quality of clot formation in whole blood based on viscoelastic blood changes | Assessment of global haemostasis Possible definition of different platelet and clotting abnormalities Diagnosis and treatment of bleeding after cardiac surgery, liver transplantation, trauma and PPH |
Platelet function tests to investigate platelet activation | ||
Flow cytometry | Cell counting, cell sorting, biomarker detection and protein engineering laser-based detection of suspending fluorescent label platelets in a stream of fluid | Expression of platelet specific surface and/or cytoplasmatic markers; VASP phosphorylation state1 (Monitoring of CD41/61, CD42, CD62P, etc. Activation markers directly dependent on thienopyridine target) |
Radio- or Enzyme Linked-Immune Assays: Soluble markers determination1 | Ligand binding assays | Measurement of Beta-thromboglobulin, PF4, GPV, Soluble P-Selectin, Thromboxanes |
- Citation: Paniccia R, Priora R, Liotta AA, Maggini N, Abbate R. Assessment of platelet function: Laboratory and point-of-care methods. World J Transl Med 2014; 3(2): 69-83
- URL: https://www.wjgnet.com/2220-6132/full/v3/i2/69.htm
- DOI: https://dx.doi.org/10.5528/wjtm.v3.i2.69