Molecular recognition of live methicillin-resistant staphylococcus aureus cells using DNA aptamers

doi:10.5528/wjtm.v2.i3.67

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World Journal of Translational Medicine

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2220-6132

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Transl Med. Dec 12, 2013; 2(3): 67-74
Published online Dec 12, 2013. doi: 10.5528/wjtm.v2.i3.67

Table 1 Quantitative representation of the different homologous families and Methicillin-resistant Staphylococcus aureus aptamer sequences after Ion Torrent sequencing and alignment

Name of aptamers	Percentage of total sequences	Sequence
DTMRSA1	2.57%	ATCCAGACGTGACGCAGC(N)₃₈TGGACACGGTGGCTTAGTA(N)₃₈ = ATGCGGTTGGTTGCGGTTGGGCATGATGTATTTCTGTG
DTMRSA2	33.74%	ATCCAGAGTGACGCAGCA(N)₃₆TGGACACGGTGGCTTA(N)₃₆ = CGACACGTTAGGTTGGTTAGGTTGGTTAGTTTCTTG
DTMRSA3	10.05%	ATCCAGAGTGACGCAGCA(N)₄₀TGGACACGGTGGCTTAGTA(N)₄₀ = GTAGATGGTTTGGTTGGTGTGGTTTCCTACTGATGTTGGG
DTMRSA4	0.32%	ATCCAGAGTGACGCAGCA(N)₃₉TGTGGACACGGTGGCTTA(N)₃₉ = TTATGGGGTGTGGTGGGGGGTTAATGCGTTGGTTATCCG

The primers used for the selection are presented in bold. Note that some primer sequences changed through the selection process.

Citation: Turek D, Simaeys DV, Johnson J, Ocsoy I, Tan W. Molecular recognition of live methicillin-resistant staphylococcus aureus cells using DNA aptamers. World J Transl Med 2013; 2(3): 67-74
URL: https://www.wjgnet.com/2220-6132/full/v2/i3/67.htm
DOI: https://dx.doi.org/10.5528/wjtm.v2.i3.67