Acetylsalicylic acid interferes with embryonic kidney growth and development by a prostaglandin-independent mechanism

Figure 3 Growth curves for cross section area of embryonic kidneys initially treated with acetylsalicylic acid followed by “rescue” treatment. Cross-section metanephroi area were used to produce growth curves of individual embryonic kidneys treated with 0.2 mg/mL (A and C) or 0.4 mg/mL (B and D) ASA from days 0-2, then rescued with either control media alone (A and B) or control media plus 10 μmol/L PGE₂ (C and D) during culture days 3-7. All embryonic kidneys in culture were compared against their contralateral controls that were exposed to control culture medium from days 0-7 (n = 8-12). ASA: Acetylsalicylic acid.