Immunofluorescence on paraffin embedded renal biopsies: Experience of a tertiary care center with review of literature

Figure 1 Examples of technically adequate and inadequate digestion. A: Immunofluorescence staining on a paraffin embedded tissue section in a case of diffuse proliferative lupus nephritis after enzymatic digestion with proteinase K. Note the adequate digestion evidenced by disappearance of tubular epithelial cells (arrow) (FITC IgG, × 200); B: Immunofluorescence staining in a case with inadequate digestion with visible tubular epithelial cells (arrow). Note the antibody sticking to the surface of the capillary wall (FITC IgG, × 200). FITC: Fluorescein isothiocyanate.