C5b-9 does not mediate tubulointerstitial injury in experimental acute glomerular disease characterized by selective proteinuria

Table 3 Markers of proliferation and apoptosis in the experimental groups

Group	n	Cortical PCNA (%)	BrdU + TECs (cells/mm2)	TUNEL + TECs (Field 1)	TUNEL + TECs (Field 4)
Control	9	0 ± 0 (0, 0-0)	0.2 ± 0.0 (0.2, 0-0.2)	6.9 ± 2.5 (8, 0-11.2)	2.7 ± 2.1 (0, 0-5.6)
PON d 2 C6+	6	0.24 ± 0.061	8.8 ± 2.71	-	-
		(0.28, 0.13-0.36)	(8.9, 2.7-15.3)
PON d 2 C6-	5	0.21 ± 0.071	6.8 ± 3.11	-	-
		(0.18, 0.14-0.30)	(6.4, 0.6-13.2)
PON d 4 C6+	6	0.34 ± 0.081	4.6 ± 0.81	-	-
		(0.30, 0.09-0.48)	(5.2, 3.4-7.4)
PON d 4 C6-	6	0.19 ± 0.041	3.2 ± 0.51	-	-
		(0.18, 0.14-0.30)	(1.2, 1.7-3.7)
PON d 8 C6+	8	0.15 ± 0.051	2.4 ± 0.71	13.4 ± 3.3	10.0 ± 2.61
		(0.11, 0.03-0.16)	(3.1, 0-4.1)	(12.8, 5.6-16.8)	(9.6, 5.6-17.6)
PON d 8 C6-	9	0.09 ± 0.031	2.0 ± 0.71	11.4 ± 3.7	7.8 ± 2.8
		(0.08, 0.02-0.11)	(1.2, 0.1-3.7)	(9.6, 0-17.6)	(6.4, 0-16)

Data are expressed as mean ± SEM (median, Inter-quartile range);

¹P < 0.05 compared to control group; Field 1 was the sub-capsular cortex; Field 4 was the inner cortex/outer medulla. PCNA: Proliferating cell nuclear antigen; TUNEL: Transferase-mediated nick end-labeling; PON: Protein-overload nephropathy; TEC: Tubular epithelial cells.