Changes in urinary excretion of water and sodium transporters during amiloride and bendroflumethiazide treatment

Figure 2 Effects of 3% hypertonic saline on urinary excretion of sodium-potassium-2chloride co-transporter (A), gamma fraction of epithelial sodium channels (B) and aquaporin2 (C) in 22 healthy subjects treated with bendroflumethiazide, amiloride or placebo. Values are means ± SEM. General linear model with repeated measurements (GLM-RM) was performed to test for differences between groups. A: U-NKCC2 increased during amiloride (P = 0.081) and placebo (P = 0.010) treatments. The increase in u-NKCC2 was however only significant during placebo. U-NKCC2 did not change during BFTZ; B: U-ENaCγ increased significantly and to the same extent during all three treatments; C: U-AQP2 increased significantly during amiloride and placebo (P < 0.001), but not during BFTZ. Paired t-test was used for comparison of post-infusion periods vs baseline. ^aP < 0.050; ^bP < 0.001. AQP2: Aquaporin2; U-NKCC2: Urinary excretion of sodium-potassium-2chloride co-transporter; ENaC: Epithelial sodium channels; BFTZ: Bendroflumethiazide.