Changes in urinary excretion of water and sodium transporters during amiloride and bendroflumethiazide treatment

Table 2 Twenty-four hours brachial blood pressure and urine collection with fluid deprivation (12 PM to 8.00 AM) in 23 healthy subjects

	Examination day			P (ANOVA)
	Thiazide	Amilorid	Placebo
Urine output (mL/24 h)	2527 ± 728	2418 ± 469	2316 ± 700	0.481
u-osm (mosm/24 h)	865 ± 158	835 ± 176	761 ± 187	0.087
CH2O (mL/min)	-0.40 ± 0.33	-0.37 ± 0.47	-0.24 ± 0.53	0.534
Cr.Cl (mL/min per m2)	113 ± 25	118 ± 32	110 ± 26	0.549
u-NKCC (ng/mmol)	0.35 ± 0.07	0.30 ± 0.05	0.32 ± 0.06	0.025
u-AQP2 (ng/mmol)	113.2 ± 39.2	103.3 ± 25.5	98.5 ± 17.4	0.244
u-ENaCγ (ng/mmol)	37.8 ± 26.7	30.8 ± 17.3	32.7 ± 15.6	0.867
u-Na (mmol/24 h)	108 ± 34	121 ± 27	106 ± 37	0.263
u-K (mmol/24 h)	80 ± 20	64 ± 21	60 ± 19	0.002
bSBP (mmHg)	119 ± 6	114 ± 7	116 ± 7	0.213
bDBP (mmHg)	72 ± 4	69 ± 3	70 ± 4	0.034

Values are means ± SD. One-way ANOVA was used for comparison between groups. P-values represent the possibility of a difference between groups. u-osm: Urine output, urine osmolality; C_H2O: Free water clearance; Cr.Cl: Creatinin clearance; u-NKCC2: Urinary NKCC2; u-AQP2: Urinary excretion of AQP2; u-ENaCγ: ENaCγ excretion adjusted for creatinin; u-Na: Urinary excretion of sodium; u-K: Potassium; bSBP: Brachial systolic blood pressure; bDBP: Brachial diastolic blood pressure.