Structural and nucleic acid binding properties of hepatitis delta virus small antigen

Figure 5 Nuclear magnetic resonance pulsed-field gradient diffusion measurements. A: NMR PFG diffusion measurements of ∆60HDAg, performed on a Bruker 600 MHz Avance II. DSS (4,4-dimethyl-4-silapentane-1-sulfonic acid) was used as a reference. ¹H pulse gradient stimulated echo longitudinal encode-decode (PG-SLED) experiment with saturation of the water signal during the relaxation delay was used with a Bruker pulse sequence: Ledbpgppr2s, at 25 °C. A 14-ppm ¹H spectral width was used. Gradient strength varied linearly from 0.963-45.7 g/cm. Solid line represents the result of fitting the experimental data to the diffusion equation (see methods); B: NMR PFG diffusion measurements of cytochrome c, previously measured on a Bruker DMX 600 MHz at 25 °C. NMR: Nuclear magnetic resonance; PFG: Pulsed-field gradient.