Evolution of an avian H5N1 influenza A virus escape mutant

Table 1 Oligonucleotides used for amplification of the H5N1 genes

Locus	Name	Primer sequence	Length	Amplicon size (bp)	Location	Ref.
N1	N1-F	ATGAATCCAAATCAGAAG	18	1350	21-38	[38]
	N1-R	TGTCAATGGTGAATGGCAAC	20		1346-1365
PB2	B2-F	GAGGCGATCTGAATTTCG	18	986	1256–1273	[39]
	B2-R	TATGCTAGAGTCCCGTTTCC	20		2222–2241
PB1	B1F	AGCGAGGAGTATCTGTGAGA	20	601	774–793	[40]
	B1R	TTCCCTCATGATTCGGTGCA	20		1356–1375
PA	PA-F	ATGAAGAGAGCAGGGCAAGA	20	868	491-510	This study
	PA-R	CAATGGGATACTTCCGCTGT	20		1339-1358
NP	NP-F	TGCTTGCCTGCTTGTGTGTA	20	665	823-842	[39]
	NP-R	TACTCCTCTGCATTGTCTCCGA	22		1466-1487
M	M-F	CCCTCAAAGCCGAAATCGCGCA	22	875	56-77	[40]
	M-R	TGCTGTTCCTGCCGATACTCTTCCC	25		906-930
NS	NS-F	CACTGTGTCAAGCTTTCAGG	20	798	23-42	[39]
	NS-R	TCTCTTGCTCCACTTCAAGC	20		786-805