Searching for nuclear export elements in hepatitis D virus RNA

Figure 7 Analysis of chloranphenicol acetyl-transferase expression in HuH-7 cells transfected with plasmids pDM138 (PRE-), pDM138 (PRE+), and pDM138 A7AS, in the absence (black columns) and presence of 10 nmol/L leptomycin B (grey columns). In order to normalize for transfection efficiency, cells were co-transfected with plasmid pSV-β-Gal (Promega). Chloranphenicol acetyl-transferase (CAT) and β-Gal expression levels were determined by enzyme-linked immunosorbent assay. Normalization of CAT expression levels was calculated by dividing the values obtained for the CAT protein by the values obtained for the β-Gal protein. The results correspond to the mean of three independent experiments. Bars represent the standard deviation.