Searching for nuclear export elements in hepatitis D virus RNA

Figure 6 Nucleo-cytoplasmic distribution of hepatitis delta virus RNA in HuH-7 cells transfected with plasmids pDL481, pDl542, pDL481ΔNEE, pDL481ΔNEEδ, and pDL481Δδ. The RNA in nuclear and cytoplasmic cell fractions was determined by real time-polymerase chain reaction using the 2^-∆∆Ct method. Results are presented as the cytoplasmic /nuclear ratio (C/N) and correspond to the mean of three independent experiments. Bars indicate the standard deviation.