Searching for nuclear export elements in hepatitis D virus RNA

Figure 5 Analysis of chloranphenicol acetyl-transferase expression in HuH-7 cells transfected with plasmids pDM138, pDM138 (PRE+), pDM138 (PRE-), pDM138 A7AS (214-417), pDM138-314-417, pDM138-214-322, pDM138-269-379, pDM138-244-379, pDM138-269-417, pDM138-214-403, pDM138-244-403, and pDM138-244-417. Chloranphenicol acetyl-transferase (CAT) and β-Gal expression levels were determined by enzyme-linked immunosorbent assay. The CAT expression values were normalized for transfection efficiency by transfecting HuH-7 cells with plasmid pSV-β-Gal (Promega) followed by determination of β-Gal expression. CAT expression values were divided by the corresponding β-Gal expression values, and the displayed results correspond to the mean of three independent experiments.