Searching for nuclear export elements in hepatitis D virus RNA

Figure 3 Analysis of chloranphenicol acetyl-transferase expression in HuH-7 cells transfected with plasmids pDM138, pDM138 (PRE+), pDM138 (PRE-), pDM138 A1S-pDM138 A10S (A), and pDM138 A1AS-pDM138 A10AS (B). In order to normalize for transfection efficiency, cells were co-transfected with plasmid pSV-β-Gal (Promega). Chloranphenicol acetyl-transferase (CAT) and β-Gal expression levels were determined by ELISA. Normalization of CAT expression levels was calculated by dividing the values obtained for the CAT protein by the values obtained for the β-Gal protein. The results correspond to the mean of three independent experiments. Bars represent the standard deviation.