Searching for nuclear export elements in hepatitis D virus RNA

doi:10.5501/wjv.v2.i3.123

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World Journal of Virology

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Virol. Aug 12, 2013; 2(3): 123-135
Published online Aug 12, 2013. doi: 10.5501/wjv.v2.i3.123

Table 2 Primers used to amplify truncated forms of the A7AS agRNA sequence

HDV cDNA fragments	Forward primer 5'→3'	Reverse primer 5'→3'	Fragment length (bp)
269-417	CGCATCGATGGGAGGAATCCACTCGGAGA	CGCATCGATTTTGCTTTCCTCCTCGCTTC	149
214-379	CGCATCGATCCCAATCCCAGATCTGGAGA	CGCATCGATGCATCTCCTCCTATCGCTATGG	166
214-403	CGCATCGATCCCAATCCCAGATCTGGAGA	CGCATCGATGCTTCGGTCTCCCCCTACTC	190
244-417	CGCATCGATCCCGAAGGGTTGAGTAGCAC	CGCATCGATTTTGCTTTCCTCCTCGCTTC	174
244-403	CGCATCGATCCCGAAGGGTTGAGTAGCAC	CGCATCGATGCTTCGGTCTCCCCCTACTC	160
314-417	CGCATCGATACCCCTTCAGCGAACAAGAG	CGCATCGATTTTGCTTTCCTCCTCGCTTC	104
214-322	CGCATCGATCCCAATCCCAGATCTGGAGA	CGCATCGATTGAAGGGGTCCTCGGAGGT	109
269-379	CGCATCGATGGGAGGAATCCACTCGGAGA	CGCATCGATGCATCTCCTCCTATCGCTATGG	111

Nucleotide sequence of the primers used to amplify eight truncated forms of the A7AS sequence localized in position 214-417 in the hepatitis delta virus (HDV) antigenomic cDNA. The underlined sequence corresponds to a ClaI restriction site.

Citation: Freitas N, Cunha C. Searching for nuclear export elements in hepatitis D virus RNA. World J Virol 2013; 2(3): 123-135
URL: https://www.wjgnet.com/2220-3249/full/v2/i3/123.htm
DOI: https://dx.doi.org/10.5501/wjv.v2.i3.123