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©2013 Baishideng.
World J Virol. May 12, 2013; 2(2): 18-31
Published online May 12, 2013. doi: 10.5501/wjv.v2.i2.18
Published online May 12, 2013. doi: 10.5501/wjv.v2.i2.18
Family | Virus | Readout | Viral life cycle step(s) studied | Partition (primary/validation) |
Flaviviridae (ssRNA+) | DENV1[100] | IF | Single step | Primary: single oligo; validation, single oligo (the same) |
Picornavirus (ssRNA+) | DCV1[22] | IF | Single step | Primary: single, validation, single (the same) |
Flaviviridae (ssRNA+) | HCV[21] | Luc (reporter virus) | Single step (replicon) | Primary: pools of 4 oligos; validation: the 4 oligos forming the pool tested individually |
Flaviviridae (ssRNA+) | HCV[20] | IF | Two step (full virus): entry/ replication; assembly/release | Primary: pools of 4 oligos; validation: the 4 oligos forming the pool tested individually |
Retroviridae (dsRNA) | HIV-1[17] | Part I: IF; part II: Luc (reporter cell line) | Two steps: entry/replication; assembly/release | Primary: pools of 4 oligos; validation: the 4 oligos forming the pool tested individually |
Retroviridae (dsRNA) | HIV-1[18] | Luc (reporter virus) | Single step | Primary only: 3 pools of 2 |
Retroviridae (dsRNA) | HIV-1[19] | Beta-Gal activity | Two steps, but without distinction between them | Primary: pools of 3; validation; independent poools of 3 |
Orthomixoviridae; (ssRNA- segmented) | INF[14] | Part I: IF; part II: Luc (reporter cell line) | Two steps: entry/replication; assembly/release | Primary: 4 oligoes |
Orthomixoviridae; (ssRNA- segmented) | INF[15] | Luc (reporter virus) | Single step | Primary only: some genes 2 oligoes, other genes only one |
Orthomixoviridae; (ssRNA- segmented) | INF1[16] | Luc (reporter virus) | Single step | Primary only: a single sirna x gene |
Rhabdoviridae (ssRNA-) | VSV[29] | IF | Single step | Primary: 2 pools of 2 oligos; validation: pool of 4 oligos from different vendor |
Flaviviridae (ssRNA+) | WNV[28] | IF | Single step | Primary: pools of 4 oligos; validation: the 4 oligos forming the pool tested individually |
- Citation: Amberkar S, Kiani NA, Bartenschlager R, Alvisi G, Kaderali L. High-throughput RNA interference screens integrative analysis: Towards a comprehensive understanding of the virus-host interplay. World J Virol 2013; 2(2): 18-31
- URL: https://www.wjgnet.com/2220-3249/full/v2/i2/18.htm
- DOI: https://dx.doi.org/10.5501/wjv.v2.i2.18