Selection of RNAi-based inhibitors for anti-HIV gene therapy

Figure 6 Competitive cell growth assay. SupT1 T cells were transduced with a short hairpin RNA (shRNA)-expressing lentiviral vector, yielding a cell population with approximately 40% GFP-positive cells. The ratio of GFP-positive cells at day 3 after transduction was set at 1 and measured longitudinally. The cells were passaged and analyzed via FACS measurement twice a week. JS1 represents the empty lentiviral vector without shRNA expression. The gray window highlights shRNAs that have a significant adverse effect on cell growth.