Interaction of immunosuppressants with HCV antivirals daclatasvir and asunaprevir: combined effects with mycophenolic acid

Figure 2 Dose-dependent inhibition of hepatitis C virus replication by daclatasvir and asunaprevir is not affected by rapamycin and tacrolimus. A, B: Huh7-ETluc cells were cultured with increasing concentrations of DCV (A) or ASV (B), in combination with different concentrations of RAPA; C, D: Huh7-ETluc cells were cultured with increasing concentrations of DCV (C) or ASV (D), in combination with different concentrations of TAC. After 24 h incubation luciferase was measured. HCV replication was effectively inhibited by ASV and DCV but not by rapamycin. RAPA and TAC had no effect on the antiviral action of ASV and DCV. Results are mean ± SE of 3 independent experiments performed in triplicate. HCV: Hepatitis C virus; DCV: Daclatasvir; ASV: Asunaprevir; RAPA: Rapamycin; TAC: Tacrolimus.