Copper as an alternative antimicrobial coating for implants - An in vitro study

Figure 5 Viability of MG-63 osteoblasts on the titanium-copper-nitride surfaces. Two different experimental arrangements were used: (A) the MG-63 cells were directly cultivated on the TiCuN surfaces for 24 h and (B) the samples were pre-incubated in DMEM for 24 h and after this the cells were seeded onto the surfaces for another 24 h. Cell viability was significantly reduced after direct seeding on TiCuN. Cell viability was higher on TiCuN + BONIT^® compared to TiCuN. Pre-incubation of the samples in DMEM for 24 h before seeding elevated cell viability on both samples (n = 3, mean value ± SD, t-test, ^dP < 0.001). TPS: Titanium plasma spray; TiCuN: Titanium-copper-nitride; DMEM: Dulbecco’s modified Eagle medium.