Islet dimension and its impact on transplant outcome: A systematic review

Table 1 Study characteristics

No	Ref.	Tissue	Source of pancreatic tissue sample	Procedure for isolation of islets	Method for islet dimension sorting
1	Farhat et al[22], 2013	Human	IIDP	Islets were maintained in CMRL 1066 medium with 2 mmol/L glutamine, 10% FBS and 1% antibiotic/anti-mycotic at 37 °C in a culture chamber with 5% CO2	Automated separation: COPAS (Union Biometrica). Manual sorting: COPAS not applicable
2	Lehmann et al[9], 2007	Human	Brain dead donors	Mechanical and enzymatic dissociation	Islets were counted, and the diameter of every islet was measured using the image analysis system. (Saisam; Microvision Instruments, Evry, France)
3	Huang and Stehno-Bittel[21], 2015	Rats	Animal	Collagenase digestion method	Manually separated by size based on the criteria
4	Vakhshiteh et al[24], 2013	Caprine (goat)	Animal	Collagenase digestion	Manually-handpicked
5	Su et al[15], 2010	Mice	Animal	Collagenase digestion	Manually sorted under the microscope
6	Kitahara and Adelman[34], 1979	Rats	Animal	Collagenase digestion	Manually under the dissection microscope
7	Sakonju et al[35], 1995	Wistar rats	Animal	Not applicable	Manually under an inverted microscope
8	Williams et al[27], 2012	Human (n = 20)	IIDP and BetaPro (Gordonsville, VA, United States)	Isolated islets were maintained in CMRL 1066 medium with 2 mmol/L glutamine, 10% FBS and 1% antibiotic/anti-mycotic at 37 °C in a culture chamber containing 5% CO2	Automated separation: COPAS (Union Biometrica). Manual sorting: COPAS not applicable
9	Steffen et al[33], 2011	Human	Donors	Collagenase digestion	Manually: Islets handpicked based on size using an eyepiece grid and sorted. Automated sorting: COPAS
10	Komatsu et al[16], 2020	Human	Human	Collagenase digestion	All islets are captured in the micrographs. Islet area measurements were converted into a 2-dimensional circular model using Cell Sens imaging software. Islet diameter calculated assuming it is spherical
11	Zorzi et al[25], 2015	C57/BL10 male mice	Animal	Collagenase digestion	Separated by size into three different groups using stainless steel mesh filtrations by gravity confirmed by light microscopy
12	Huang et al[28], 2011	Rats	Animal	Collagenase digestion	Automated separation: COPAS (Union Biometrica). Manual sorting: COPAS not applicable
13	Cui et al[36], 2005	Syrian golden hamster	Animal	Collagenase digestion	Manual sorted
14	Williams et al[29], 2010	Rats	Animal	Collagenase digestion	Manually sorted. Automated: COPAS (Union Biometrica, Holliston, MA)
15	Li et al[17], 2014	Rats	Animal	Collagenase digestion	Manually sorted
16	MacGregor et al[23], 2006	Rats	Animal	Collagenase digestion	Sedimentation method
17	Hopcroft et al[32], 1985	Wistar rats	Animal	Collagenase digestion	Manually under an inverted microscope
18	Warnock et al[31], 1988	Human	Human	Collagenase digestion	Manually under stereomicroscope
19	Aizawa et al[30], 2001	Wistar rats	Animal	Collagenase digestion	Manually under microscope

COPAS: Complex Object Parametric Analyzer Sorter; IIDP: Integrated islet distribution program.