Haemoadsorption cartridge connected to the machine perfusion for donation after circulatory death porcine liver marginal grafts

Figure 4 Representative hematoxylin and eosin staining histology of liver tissue at the hepatic injury score at different time points during machine perfusion treatment with or without Persorb device. Liver biopsies were analyzed, thereby retrieved at the slaughterhouse immediately after the donor warm ischemia time: Liver biopsies retrieved at the slaughterhouse showed low/absent liver damage. At start of liver biopsies, collected after long-time of cold static storage in ice showed diminished liver architecture, with sinusoid dilation, congestion, portal inflammation, microvacuolization and infiltrating inflammatory cells. The hepatocytes appeared swollen and the hepatic cords were disorganized, furthermore exhibited patchy subcapsular hepatocyte necrosis (black arrows). After hypothermic machine perfusion (HMP) treatments, repaired interlobular septa of pig livers and improved tissue architecture were detected. Significant histological alteration were detected at the end in HMP and HMP-Sorb group. All these results were graphically summarized with the trend of the hepatic injury score. ^aP < 0.05. HMP: Hypothermic machine perfusion; NoSorb: Hypothermic machine perfusion without the cartridge; Sorb: Hypothermic machine perfusion with PerSorb; Tend: At the end; T-1: Liver biopsies were analyzed, thereby retrieved at the slaughterhouse immediately after the donor warm ischemia time; T0: Start; T2: After two hours.