Cell mediated gene therapy: A guide for doctors in the clinic

Table 2 Different types of production methods based on cells needed per batch

Cells/lot (billions)	Cell culture	Harvest	Filling	Freezing
1	T175 flask or hyperflask, 10 layer cell factory	Bucket centrifugation	Hand/manual fill	Bench top control rate freezer
10	10 layer cell factory, hyperstack-12, Xpansion systems	Bucket centrifugation	Semi automated fill machine or hand/manual fill	Bench top control rate freezer
50	Hyperstack or Xpansion, cell cube	Tangential flow filtration, continuous centrifugation	Automated fill machine	Large scale control rate freezer
100	Factory automation of Xpansion or Hyperstack technologies	Tangential flow filtration of continuous centrifugation	Automated fill machine	Large scale control rate freezer
500	Bioreactors using microcarriers	Continuous centrifugation	Automated fill machine	Scale out large scale control rate freezer