An inhibitor of HIF-α subunit expression suppresses hypoxia-induced dedifferentiation of human NSCLC into cancer stem cell-like cells

Figure 7 Hypoxia-inducible factor mediates the expression of differentiation- and stem cell-related genes in A549 cells. A and B: Effect of DFO, the cells were cultured with or without 100 μmol/L DFO for 3 d; C and D: Effect of CoCl₂, the cells were cultured in the presence or absence of 200 μmol/L CoCl₂ for 3 d; E and F: Effect of the ectopic expression of HIF-1α^P402A/P564A, the cells were transfected with pcDNA3.1 or pcDNA3.1/HIF-1α^P402A/P564A and allowed to grow for 2 d; G and H: Effects of TSA and 5-azacytidine on the hypoxia-induced repression of SP-C expression, the cells were cultured under normoxic (N) or hypoxic (H) conditions in the presence or absence of TSA (300 nmol/L) or 5-azacytidine (5-azaC) (4 μmol/L) for 3 d. B, D, F, and H: The expression level of each gene was normalized to that of β-Actin (ACTB), data are shown as fold-change relative to normoxia (normoxia values set to equal 1).