An inhibitor of HIF-&alpha; subunit expression suppresses hypoxia-induced dedifferentiation of human NSCLC into cancer stem cell-like cells

doi:10.5496/wjmg.v3.i4.41

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World Journal of Medical Genetics

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2220-3184

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Med Genet. Nov 27, 2013; 3(4): 41-54
Published online Nov 27, 2013. doi: 10.5496/wjmg.v3.i4.41

Table 1 Primers used for reverse transcription-polymerase chain reaction analyses

Gene	Forward primer	Reverse primer	Accession #
CC10	CTTTCAGCGTGTCATCGAAA	TTGAAGAGAGCAAGGCTGGT	U01101
SP-C	TCCACATGAGCCAGAAACAC	CTGGCCCAGCTTAGACGTAG	NM_003018
AQP5	CTACTTCACTGGCTGCTCCA	GTGGTCAGCTCCATGGTCTT	NM_001651
CD133	TGGAGTGCAGCTAACATGAG	TGCACATGAAAAGACCTGGG	NM_006017
4-Oct	GAGGAGTCCCAGGACATCAA	CTCCAGGTTGCCTCTCACTC	NM_002701
MSI1	GTTCAGAGCGTTGGACCTTC	AAACCCAAAACACGAACAGC	NM_002442
GLDC	ATTTCTCGTTGATCCCCGTTGC	GCGATGTCTACCCCAAATTCTC	NM_000170
NANOG	ACCAGACCCAGAACATCCAG	TTCACACGTCTTCAGGTTGC	NM_024865
NES	AACAGCGACGGAGGTCTCTA	TTCTCTTGTCCCGCAGACTT	NM_006617
BMI1	AGAGCTGGAAGTCGAGTGT	GCACCTTCACATTCCTCTC	NM_005180
EGFR	GCACGAGTAACAAGCTCACG	TTCCTCTGATGATCTGCAGG	NM_005228
ERBB2	AGCAGAGGATGGAACACAGCGG	CTCCTGGATATTGGCACTGG	NM_004448
NOTCH1	TGCTGGACGAGTACAACCTG	CGCATTGACCATTCAAACTG	AF308602
NOTCH2	ACCCTTGTGAGAATGCTGCT	CCATACCACTGAAGCCTGGT	NM_024408
CD117	CTATGCTCTCGCACCTTTCC	CAATGAAGTGCCCCTGAAGT	X06182
MET	GGTTTTTCCTGTGGCTGAAA	GGCATGAACCGTTCTGAGAT	NM_001127500
CD34	ACAACACGTGGTGGCTGATA	GAGTTTACCTGCCCCTCCTC	NM_001773
CD44	TGGAGCAAACACAACCTCTG	TCCACTTGGCTTTCTGTCCT	NM_000610
CD45	AGATGCCCAGTGTTCCAC	AGGGTTGAGTTTTGCATTGG	NM_002838
PCGF2	TTGCAGTGGAAACTTTGTGC	AGGTGAGACTCCACCACCAG	NM_007144
CDH1	GGTTATTCCTCCCATCAGCT	CTTGGCTGAGGATGGTGTA	L08599
SOX2	CCCCCCTGTGGTTACCTCTTC	TTCTCCCCCCTCC AGTTCG-3	NM_003106
RUNX3	GCTGTTATGCGTATTCCCGTAG	TGAAGTGGCTTGTGGTGCTGAGTGA	NM_001031680
ACTB	TGACGGGGTCACCCACACTGTGCCCATCTA	CTAGAAGCATTTGCGGTGGACGATGGAGGG	NM_001101

GLCD: Glycine decarboxylase.

Citation: Akimoto M, Nagasawa H, Hori H, Uto Y, Honma Y, Takenaga K. An inhibitor of HIF-α subunit expression suppresses hypoxia-induced dedifferentiation of human NSCLC into cancer stem cell-like cells. World J Med Genet 2013; 3(4): 41-54
URL: https://www.wjgnet.com/2220-3184/full/v3/i4/41.htm
DOI: https://dx.doi.org/10.5496/wjmg.v3.i4.41