Antioxidant enzyme profile of two clinical isolates of Entamoeba histolytica varying in sensitivity to antiamoebic drugs

Table 1 Primers used in RT-PCR of Entamoeba histolytica thioredoxin reductase, peroxiredoxin, FeSOD and 18SrRNA

S.No.	Primer	Sequence	Tm	Amplicon size	Ref.
1	Thioredoxin reductase (TrxR)	F-5’GTAATATTCATGATGTTGT3’	48 °C	204 bp	[4]
	Accession no (EHI_155440)	R-5’CATCATTAATTCATTTTCCA3’	48 °C
2	Eh Peroxiredoxin (Prx)	F 5’AAATCAATTGTGAAGTTATTGG3’	53.6 °C	100 bp	[16]
		R 5’TCCTACTCCTCCTTTACTTTTA3’	56.8 °C
3	FeSOD	F 5’ACAATTACCTTATGCTTATAA3’	52 °C	240 bp	[16]
	Accession number (XM_643735.2)	F 5’TCCACATCCACACATACAAT3’	54 °C
4	Entamoeba histolytica 18s ribosomal RNA gene	F 5’TCAGCCTTGTGACCATACTC3’	61.7 °C	200 bp	[16]
		F 5’AAGACGATCAGATACCGTCG3’	68.9 °C