Natural contamination of human hands with enteric parasites in Indian Subcontinent

Table 1 Polymerase chain reaction primers used in this study

Target parasite	Target gene	Primer	Primer sequence (5’-3’)	Annealingtemperature	PCR Product size (bp)	Ref
Giardia lamblia	Beta-giardin	MAH433F	CATAACGACGCCATCGCGGCTCTCAGGAA	60	218	Rochelle et al[19]
		MAH592R	TTTGTGAGCGCTTCTGTCGTGGCAGCGCTAA
Ascaris lumbricoides	rDNA	ITS-1F	TGCACATAAGTACTATTTGCGCGTAT	60	82	Pecson et al[20]
		ITS-1R	TGATGTAATAGCAGTCGGCGG
Entamoeba histolytica	SSU rRNA	EH1	GTACAAAATGGCCAATTCATTCAATG	51	128	Gonin et al[21]
		EH2	ACTACCAACTGATTGATAGATCAG
Cryptosporidium sp.	SSU rRNA	18 SF	TTCTAGAGCTAATACATGCG	55	1325	Xiao et al[32]
		18 SR	CCCTAATCCTTCGAAACAGGA
Cryptosporidium sp.	Nested PCR for SSU rRNA		GAAGGGTTGTATTTATTAGATAAAG	55	825	Xiao et al[32]
			AAGGAGTAAGGAACAACCTCCA

PCR: Polymerase chain reaction.