GadE regulates fliC gene transcription and motility in Escherichia coli

Table 4 Assessing a gadE and mutation and complementation on fliC::lacZ gene transcription in Escherichia coli grown in buffered pH 5.5 and 7.0 Luria Bertani media with different osmolarities

E. coli strain	Gal activity1
	pH 5.5	pH 5.52	pH 7.0	pH 7.0
EK227/pNK2-29	540 ± 513	165 ± 59	1520 ± 144	540 ± 66
EF1007/pNK2-294	1742 ± 109	470 ± 106	2196 ± 173	681 ± 135
EF1083/pNK2-29	295 ± 93	131 ± 20	794 ± 145	404 ± 41

¹Galactosidase activity measured as Miller units.

²400 mmol/L added NaCl.

³Data represents the mean ± standard deviation from three separate runs.

⁴EF1007 is gadE and EF1083 is gadE/pGadE+.