GadE regulates fliC gene transcription and motility in Escherichia coli

Table 1 Bacterial strains and plasmids used in the study

Strain/plasmid	Description	Source
Strain
DH5a MCR	Transformation efficient strain	Gibco/BRL
MC4100	E. coli K-12 strain	Linda Kenney
EK227	E. coli K-12 strain	[53]
EF1007	gadE::Km in EK227	[54]
EF1083	gadE::Km/pPCRScript Amp gadE	[33]
NU149	Clinical isolate	[36]
NU149 gadE	ΔgadE mutation in NU149	This study
NU149 LacZ1	ΔlacZ mutation in NU149	This study
Plasmid
pUJ9	Promoterless lacZ gene, ApR	[37]
pPP2-6	Single copy plasmid, CmR	[38]
pKD4	Flp recombinase sites, KmR	[40]
pKD46	Red recombinase, ApR	[40]
pCP20	Flp recombinase, ApR	[40]
pNK2-29	fliC::lacZ on pPP2-6, ApR	This study
pPCRScript Amp gadE	gadE on pPCRScript Amp	[33]