Prevalence of RUNX1 gene alterations in de novo adult acute myeloid leukemia

Table 3 Association between runt-related transcription factor-1 amplifications and runt-related transcription factor-1 deletion with clinicopathological features of acute myeloid leukemia patients

Parameter		RUNX1 amplification		P value	RUNX1 deletion		P value
		Negative	Positive		Negative	Positive
Age in years, median (IQR)		42.0 (18-70)	36.0 (20-78)	0.694	41.5 (18-78)	21.5 (21-22)	0.175
TLC as × 109/L, median (IQR)		44.7 (1-377)	19.2 (2-91)	0.873	29.1 (1-377)	105.3 (19-191)	0.630
Hb in g/dL, mean ± SD		8.33 ± 2.50	7.70 ± 1.70	0.359	8.20 ± 2.40	8.10 ± 1.50	0.950
Platelets as × 109/L, median (IQR)		24 (12-226)	45 (21-185)	0.484	26 (12-226)	24 (18-30)	0.663
PB blast (%), median (IQR)		60 (0-99)	38 (5-72)	0.515	59 (0-99)	47 (40-54)	0.232
BM blast (%), median (IQR)		66 (30-97)	60 (20-90)	0.35	65.5 (20-97)	74.5 (71-78)	0.613
MCN, mean ± SD		45.4 ± 2.5	47.5 ± 2.4	0.007	46 ± 2.1	42.5 ± 7	0.008
Sex, n (%)	Male	34 (56.7)	13 (76.5)	0.168	45 (61.6)	2 (50.0)	0.641
	Female	26 (43.3)	4 (23.5)		28 (38.4)	2 (50.0)
BM cellularity, n (%)	Hypocellular	1 (1.7)	2 (11.8)	0.137	3 (4.1)	0 (0.0)	0.626
	Normocellular	8 (13.3)	3 (17.6)		11 (15.1)	0 (0.0)
	Hypercellular	51 (85.0)	12 (70.6)		59 (80.8)	4 (100.0)
Hepatomegaly, n (%)	Absent	47 (78.3)	10 (58.8)	0.125	53 (72.6)	4 (100.0)	0.568
	Present	13 (21.7)	7 (41.2)		20 (27.4)	0 (0.0)
Splenomegaly, n (%)	Absent	51 (85.0)	9 (52.9)	0.009	56 (76.7)	4 (100.0)	0.57
	Present	9 (15.0)	8 (47.1)		17 (23.3)	0 (0.0)
Lymphadenopathy, n (%)	Absent	44 (73.3)	8 (47.1)	0.076	49 (67.1)	3 (75.0)	0.743
	Present	16 (26.7)	9 (52.9)		24 (32.9)	1 (25.0)
MPO, n (%)	Negative	6 (10.0)	2 (11.8)	0.833	8 (11.0)	0 (0.0)	1
	Positive	54 (90.0)	15 (88.2)		65 (89.0)	4 (100.0)
CD34, n (%)	Negative	34 (56.7)	8 (47.1)	0.584	38 (52.1)	4 (100.0)	0.121
	Positive	26 (43.3)	9 (52.9)		35 (47.9)	0 (0.0)
CD64, n (%)	Negative	45 (75.0)	12 (70.6)	0.758	56 (76.7)	1 (25.0)	0.052
	Positive	15 (25.0)	5 (29.4)		17 (23.3)	3 (75.0)
CD14, n (%)	Negative	51 (85.0)	17 (100.0)	0.194	66 (90.4)	2 (50.0)	0.065
	Positive	9 (15.0)	0 (0)		7 (9.6)	2 (50.0)
FAB, n (%)	M0	2 (3.3)	2 (11.8)	0.368	4 (5.5)	0 (0.0)	0.014
	M1	6 (10.0)	4 (23.5)		10 (13.7)	0 (0.0)
	M2	32 (53.3)	6 (35.3)		38 (52.1)	0 (0.0)
	M4	17 (28.3)	5 (29.4)		19 (26.0)	3 (75.0)
	M5	2 (3.3)	0 (0)		1 (1.4)	1 (25.0)
	M7	1 (1.7)	0 (0)		1 (1.4)	0 (0.0)
Complex, n (%)	Negative	52 (94.5)	8 (47.1)	0.001	57 (87.7)	3 (75.0)	0.436
	Positive	3 (5.5)	6 (35.3)		8 (12.3)	1 (25.0)
t (8; 21), n (%)	Negative	54 (90.0)	16 (94.1)	0.602	66 (90.4)	4 (100.0)	0.516
	Positive	6 (10.0)	1 (5.9)		7 (9.6)	0 (0.0)
inv16, n (%)	Negative	55 (91.7)	16 (94.1)	0.739	68 (93.2)	3 (75.0)	0.282
	Positive	5 (8.3)	1 (5.9)		5 (6.8)	1 (25.0)
t (9; 22), n (%)	Negative	59 (98.3)	16 (94.1)	0.395	71 (97.3)	4 (100.0)	0.737
	Positive	1 (1.7)	1 (5.9)		2 (2.7)	0 (0.0)
Cytogenetic risk, n (%)	High	12 (20.0)	5 (29.4)	0.15	15 (20.5)	2 (50.0)	0.288
	Intermediate	31 (51.7)	11 (64.7)		40 (54.8)	2 (50.0)
	Low	17 (28.3)	1 (5.9)		18 (24.7)	0 (0.0)
FLT3-ITD, n (%)	Wildtype	52 (86.7)	17 (100.0)	0.188	66 (90.4)	3 (75.0)	0.361
	Mutant	8 (13.3)	0 (0)		7 (9.6)	1 (25.0)
C-KIT, n (%)	Wildtype	59 (98.3)	17 (100.0)	0.592	72 (98.6)	4 (100.0)	0.814
	Mutant	1 (1.7)	0 (0)		1 (1.4)	0 (0.0)
NPM, n (%)	Wildtype	47 (78.3)	16 (94.1)	0.173	60 (82.2)	3 (75.0)	0.717
	Mutant	13 (21.7)	1 (5.9)		13 (17.8)	1 (25.0)
BM cellularity on day 28, n (%)	Hypocellular	6 (20.0)	0 (0)	0.152	5 (12.8)	1 (25.0)	0.22
	Normocellular	13 (43.3)	8 (47.1)		21 (53.8)	0 (0.0)
	Hypercellular	11 (36.7)	3 (25.0)		13 (33.3)	1 (25.0)
CR, n (%)	Negative	29 (48.3)	8 (47.1)	0.926	36 (49.3)	1 (25.0)	0.616
	Positive	31 (51.7)	9 (52.9)		37 (50.7)	3 (75.0)
Delayed CR, n (%)	Negative	56 (93.30	14	0.177	66 (90.4)	4 (100.0)	0.516
	Positive	4 (6.7)	3 (25.0)		7 (9.6)	0 (0.0)
Relapse, n (%)	Negative	50 (83.3)	13	0.496	61 (83.6)	2 (50.0)	0.149
	Positive	10 (16.7)	4		12 (16.4)	2 (50.0)
Death, n (%)	Negative	13 (21.7)	7	0.125	19 (26.0)	1 (25.0)	0.964
	Positive	47 (78.3)	10		54 (74.0)	3 (75.0)
Early death, n (%)	Negative	30 (50.0)	11	0.41	39 (53.4)	2 (50.0)	0.894
	Positive	30 (50.0)	6		34 (46.6)	2 (50.0)

BM: Bone marrow; CR: Complete remission; FAB: French-American-British; Hb: Hemoglobin; IQR: Interquartile range; MCN: Modal chromosomal number; PB: Peripheral blood; MPO: Myeloperoxidase; RUNX1: Runt-related transcription factor-1; SD: Standard deviation; TLC: Total leucocytic count.