Isolation and identification of adipose-derived stromal/stem cells from breast cancer patients after exposure neoadjuvant chemotherapy

Figure 5 Recovery of adipose-derived stromal cells analysis following exposure to individual chemotherapeutic agents in vitro human population. A: ASCs were isolated from female patients during reconstructive procedures. Cell viability was measured by MTT assays after ASCs treated with different dosages of 5-FU, DXR and cyclophosphamide on 24, 48, and 72 h. B: Growth curves of ASCs treated with 5-FU or DXR for 72 h then removed and the cells were cultured in medium without drugs for an additional 8 d. The results represent the mean ± SE of triplicate cultures of three representative experiments. n = 3, ^aP < 0.05 vs control. 5-FU: 5-fluorouracil; DXR: Doxorubicin.