Isolation and identification of adipose-derived stromal/stem cells from breast cancer patients after exposure neoadjuvant chemotherapy

Figure 4 Adipogenic differentiation capacity of adipose-derived stromal cells in patients after received neoadjuvant chemotherapy treatment. A: Oil red O stains in the adipogenic-induced ASCs from the cancerous side of the breast (CA-ASC) and noncancerous side of the breast (N-ASC) in patients receiving NAC treatment (+NAC) vs not-receiving NAC (-NAC) from undifferentiated ASCs (left) and differentiated ASCs (right). B: Quantified by extracting the dyes for evaluating the adipogenic differentiated cells levels from the cancerous side of the breast or noncancerous side of the breast ASCs in patients receiving NAC treatment vs not-receiving NAC. C: Real-time PCR evaluating the mRNA expression of peroxisome proliferator activated receptor gamma (PPARγ) and lipoprotein lipase (LPL) in differentiating ASCs from the cancerous side of the breast or noncancerous side of the breast in patients receiving NAC treatment vs not-receiving NAC (n = 21, mean ± SE, P = NS). ASC: Adipose-derived stromal cell; NAC: Neoadjuvant chemotherapy.