Isolation and identification of adipose-derived stromal/stem cells from breast cancer patients after exposure neoadjuvant chemotherapy

Figure 1 Experimental design. Adipose-derived stromal cells (ASCs) were isolated from both the cancerous side and noncancerous side of the breast from the same patients with receiving neoadjuvant chemotherapy (NAC) treatment or not-receiving NAC. The number of ASCs yield, proliferation rates and the ability to undergo adipogenic differentiation was analyzed by constructing growth curves, quantitative RT-PCR and histological staining and compared to the ASCs obtained from patients not-receiving NAC treatment. ASCs: Adipose-derived stromal cells; NAC: Neoadjuvant chemotherapy.