Surgical and immune reconstitution murine models in bone marrow research: Potential for exploring mechanisms in sepsis, trauma and allergy

Figure 3 Main steps of the intraperitoneal modification of the egg white implant procedure. A: Mice are anesthetized with i.p. administration of ketamine (100 mg/kg) and xylazine (12 mg/kg); B: After local asepsis, a cut is made in the skin and in the peritoneal membrane, providing access to the peritoneal cavity; C: A pellet of heat-coagulated egg white is placed through the opening in the peritoneal cavity; D: The peritoneal membrane and the skin are sutured and the animals are placed to recover from anesthesia with care to avoid hypothermia and corneal dryness or undue exposure to direct light. For sham-implanted controls, step C is omitted.