Lipofuscins prepared by modification of photoreceptor cells via glycation or lipid peroxidation show the similar phototoxicity

Figure 3 Production of model lipofuscin in the reaction between modified photoreceptor outer segments and bisretinoid A2E. A: A2E binding by modified photoreceptor outer segments leads to a change in their fluorescence characteristics: curve 1 - POS modified oxidized prior to incubation with A2E; curve 2 - modified POS containing A2E (LPO - lipofuscin); B: Comparison of fluorescence characteristics of the model lipofuscin (LPO) and natural lipofuscin granules from human RPE: curve 1 (solid line) - LPO; curve 2 (dotted line) - lipofuscin granules from human RPE. Model lipofuscin preparation is described in materials and methods, excitation wavelength - 365 nm. POS: Photoreceptor outer segments; RPE: Retinal pigment epithelium.