Epigallocatechin-3-gallate suppresses transforming growth factor-beta signaling by interacting with the transforming growth factor-beta type II receptor

Figure 2 Effects of scavenging compounds on expression of α-smooth muscle actin. MRC-5 cells were treated with edaravone (100 μmol/L) (A) or N-acetylcysteine (NAC) (2.5 mmol/L) (B) for 1 h, and then stimulated with transforming growth factor-β (TGF-β) for 24 h. Cell lysates were electrophoresed, blotted onto Immobilon, and probed with anti-α-smooth muscle actin (α-SMA). GAPDH: Glyceraldehyde 3-phosphate dehydrogenase. FBS: Fetal bovine serum.