Potential roles of longan flower and seed extracts for anti-cancer

Figure 2 Cell cycle analysis of longan seed extract -treated cancer cells. About 1 × 10⁶ cells in 10 mL medium in 100 mm plate were treated with increasing concentrations of longan seed extract as indicated, then incubated at 37 °C for 48 h. The (A) A-549, (B) Hep G2, (C) C-33A and (D) MDA-MB-231 cells harvested by trypsinization were fixed in 70% alcohol at -20 °C for 2 h and then reconstituted in phosphate-buffered saline. The cells were stained with propidium iodide solution in the dark at room temperature for 30 min. The stained cells were then analyzed using the FL-2A parameter of a flow cytometer to obtain the DNA content of the cells, and the distribution in each cell-cycle phase was determined using Modfit software. Data are expressed as a percentage of the total cells. Data represent the average of three independent experiments, and are expressed as the mean ± SD. ^aP < 0.05.